基于微立体光刻技术的组织支架的制备

介绍了基于微立体光刻技术制造的三维支架初步模型.由微立体光刻设备制备出了μm级的圆柱阵列式支架和三角孔式支架,整体结构很微小,结构上端为原生代细胞附着区域,下端是细胞培养液的贮液池.支架结构精度较高,可以达到传统快速成型技术不能具备的等级,其微孔之间的孔隙结构达到200μm左右,同时,微孔的数量、大小、分布及形状可人工影响或控制.姜姜     

Velocity and myosin phosphorylation transients in arterial smooth muscle: effects of agonist diffusion

Summary Transients in myoplasmic [Ca²⁺] and in phosphorylation of the 20,000 dalton light chain of myosin have been reported following stimulation of vascular smooth muscle by various agonists. Since these transients are rapid compared with the time required to attain a steady-state stress, agonist diffusion rates may be a significant limitation in activation. The purpose of this study was to estimate the effect of agonist diffusion rates on the time course of activation as assessed by mechanical measurements of stress development and isotonic shortening velocities and by determinations of the time course of myosin phosphorylation. The approach was to measure these parameters in K⁺-stimulated preparations of the swine carotid media of varying thicknesses and to estimate the theoretical contributions imposed by diffusion rates and the presence of a diffusion boundary layer surrounding the tissue. The results show that the time course of parameters which are tissue averages such as stiffness, active stress, and myosin phosphorylation is dominated by agonist diffusion rates. The sequence of events involved in excitation-contraction coupling including agonist actions on the cell membrane, Ca²⁺ release, activation of myosin light chain kinase, and cross-bridge phosphorylation appear to be very rapid events compared with stress development. Estimates of unloaded or lightly loaded shortening velocities which are not simple tissue averages appear to provide an imporoved estimate of activation rates.Supported by a grant from the National Institutes of Health 5-PO1-HL19242. K. E. Kamm was supported by a National Heart, Lung, and Blood Institute Research Service Award HL-05957.     

Mechanical and biochemical characterization of the contraction elicited by a calcium-independent myosin light chain kinase in chemically skinned smooth muscle

Summary The contraction induced by a Ca²⁺-independent myosin light chain kinase (MLCK-) was characterized in terms of isometric force (F_o), immediate elastic recoil (SE), unloaded shortening velocity (V_us), shortening under a constant load and ATPase activity of chemically skinned smooth muscle preparations. These parameters were compared to those measured in a Ca²⁺-induced contraction to assess the nature of cross bridge interaction in the MLCK-induced contraction. F_o developed in chicken gizzard fibers as well as SE were similar in contractions elicited by either agent. V_us in the contraction induced by MLCK-(0.36 mg/ml) was similar though averaged 39.3±8.9% less than V_us induced by Ca²⁺ (1.6x10^–6M) in the control fibers. Addition of Ca²⁺ (1.6x10^–6M) to a contraction induced by MLCK-resulted in small increases in both F_o and V_us. Shortening under a constant load was similar for both types of contractions. The contraction induced by MLCK-was accompanied by an increased rate of ATP hydrolysis. The MLCK-induced contraction is thus kinetically similar though not identical to a contraction induced by Ca²⁺. We conclude that with respect to actin-myosin interaction, MLCK- and Ca²⁺-induced contractions are similar.     

利用偏光、稀释显影法实现黑白图象的假彩色显示

白偏光照射高分辨率干板,经稀释显影后呈现出负二向色性。本文提供了利用二向色性实现黑白图象的假彩色编码和图象相减的一种新方法,给出了实验结果。     

LED冷光源在矿山企业的应用

阐述了LED冷光源的发展概况及其与传统光源相比的优点,并根据现阶段山西嘉士朗科技公司设计制造的KL4ML(A)型矿灯和正在研发的DGS18/127L(A)型巷道灯等系列产品为例,说明了LED冷光源在矿山企业的应用。     

基于80C51单片机的智能电动小车

采用80C51单片机为控制核心,利用超声波传感器检测道路上的障碍,控制电动小汽车的自动避障,快慢速行驶,以及自动停车,并可以自动记录时间、里程和速度,自动寻迹和寻光功能。整个系统的电路结构简单,可靠性能高。     

宾主盒的光稳定性

本文研究了宾主相互作用的液晶彩色显示盒中二向色性染料的光稳定性。发现宾主盒吸光度的减少几乎正比于暴光时间和强度。经加速寿命试验得到染料的实际寿命,其中若干种蒽醌染料的实际寿命超过十年;在吸光度从A_0(t=0)到0.8A_0范围内,染料在液晶中的有序参数在光照前后几乎不变;探讨了染料分子结构与光稳定性之间的关系。     

论光影设计中的黑与白

文章针对黑色与白色进行举例，介绍了两者在宇宙及其现代人们生活中的关系。     

液晶光阀的电光频率特性

测量并分析了TB3639型液晶光阀的电光特性曲线,讨论了频率变化对液晶显示器显示稳定性的影响.结果表明:阈值电压和饱和电压都随频率的增大而逐渐增大,尤其是阈值电压受频率影响更大,频率对陡度也有较大的影响.而阈值电压的变化与陡度的变化会影响LCD显示的稳定性,阈值电压升高,显示对比度会下降,阈值电压降低,易产生交叉串扰现象.经分析得出:在60～100 Hz区间,所测试的液晶光阀陡度随频率变化相对较小,显示特性较稳定.这为设计液晶显示器件,提高其显示稳定性提供了依据.     

糖浆浓度激光自动检测过程的数理分析

通过对糖浆浓度激光自动检测过程的机理分析，以糖浆过饱和度的一般定义为依据，对糖浆浓度与折射率、折射率与临界角、临界角与亮区长度以及亮区长度与像元数的关系等进行了数学描述，最后导出了测量过程的数学模型．